Expression of miRs 202-3p, 29a, and 21 in exosomes derived from plasma of CLL patients versus normal donors.

<p><b>(A)</b> Exosomes sourced from plasma obtained from CLL patients or healthy donors (n = 3 each) were subjected to RT-qPCR analysis for absolute levels of miR-202-3p using a standard curve method. The levels of miR-202-3p are significantly higher in exosomes purified from CLL plasma (p = 0.03). <b>(B)</b> Absolute levels of miR-29a and miR-21 in exosomes harvested from CLL patient and healthy donor plasma (n = 3) were determined by RT-qPCR analysis. Standard curves for miR-29a and miR-21 were generated using the miRVana^TM miRNA reference panel v9.1. Statistical analysis was performed using an unpaired t-test and yielded <i>p values</i> of 0.01 and 0.2 for miR-29a and miR-21 respectively.