Mutational analysis for the β2 adrenergic receptor.

<p>(A) Workflow schematic of the mutational analysis for the β2 adrenergic receptor. First step was to reduce the primary sequence of the mouse β2AR (mβ2AR) to that of the non-trafficking odorant receptor M71. Then we asked what is the C-terminus minimal requirement for trafficking and activity by analyzing Ct truncations series as well as Ct full deletion. Furthermore we analyzed the effects of mutating a putative retention signal that might be revealed by a Ct deletion. We also compared the phenotype of mouse and human β2AR activity mutants to M71. Finally, we analyzed the behavior of M71/mβ2AR chimera. (B) The 2D topology of mouse β2AR was generated using the online software Topo2 (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0141696#sec002" target="_blank">Material & Methods</a>). Plasma membrane is symbolized by two horizontal lines with the extracellular compartment on top and intracellular compartment on the bottom. Putative transmembrane domains are located in between those two lines. Different shapes and colors are used to symbolize the residues targeted for mutations. N-linked glycosylation sites are indicated in black boxes and their N in diamonds.