Overlay of the Ribbon Structure apo-CobY (cyan) with those of Structurally Similar Proteins.

<p>(A) (green) X-ray structure of the CobY^G153D:GTP complex (PDB 3RSB) (87% structural overlap with rmsd 1.82 Å; fragment/topology score 0.95/0.94 with match size 113). (B) (gray) X-ray structure of CMP:2-keto-3-deoxy-manno-octonic acid synthetase (PDB 1H7F).(75.5% structural overlap with rmsd 1.84 Å; fragment/topology score 0.93/1.0 with match size 148). (C) (gray) X-ray structure of α-D-glucose-1-phosphate cytidylyl transferase (PDB 1WVC) (75.5% structural overlap with rmsd 2.15 Å; fragment/topology score 0.86/1.0 with match size 148). (D) (gray) X-ray structure of acytidylyl transferase (PDB 2VSI) (74.5% structural overlap with rmsd 1.98 Å; fragment/topology score 0.91/1.0 with match size 146). (E) X-ray structure of 2-C-methyl-D-erythritol 4-phosphate cytidylyl transferase(PDB 1VPA) (73% structural overlap with rmsd 1.86 Å; fragment/topology score 0.91/1.0 with match size 143). (F) X-ray structure of CMP-acylneuraminate synthetase (PDB 1EYR) (70% structural overlap with rmsd 1.95 Å; fragment/topology score 0.88/1.0 with match size 137). PYMOL [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0141297#pone.0141297.ref020" target="_blank">20</a>] was used to generate the structures, and the CLICK [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0141297#pone.0141297.ref033" target="_blank">33</a>] program was used to carry out the pairwise alignments.