Over-expression of proteasome β1 subunit increased sensitivity of cells to cytotoxicity of fangchinoline.

<p>(A) Results of Western blotting assay of proteasome β1 subunit protein expression in wild-type cells, negative-control cells and cells transfected with plasmid encoding PSMB6. (B) Cell viability of wild-type cells, negative-control cells and cells transfected with plasmid encoding PSMB6 after 24 h treatment of fangchinoline at different concentrations. (C) Cell viability of wild type cells, negative control cells and cells transfected with plasmid encoding PSMB6 after 48 h fangchinoline treatment. (d) Cell viability of wild type cells, negative control cells and cells transfected with plasmid encoding PSMB6 after 72 h fangchinoline treatment. Data were statistical results of three independent experiments.