Knockdown of proteasome β1 subunit ameliorated cytotoxicity of fangchinoline in PC-3 cells.

<p>(A) Results of Western blotting assay of proteasome β1 subunit protein expression in wild-type cells, negative-control cells and PSMB6 knockdown cells (cells transfected with siRNAs for PSMB6). (B) Cell viability of negative-control cells and cells transfected with siRNAs for PSMB6 after 72 h treatment of fangchinoline at different concentrations. Data were statistical results of three independent experiments. (C) Effects of fangchinoline treatment on markers of apoptosis and autophagy in negative control cells or cells transfected with siRNAs for PSMB6. Showed were representative results of three independent experiments.