AKP-11 withdrawal increases cell surface S1P1 receptor expression.

<p>(A-B) CHO cells expressing S1P1-HA were pretreated with cycloheximide (15μg/ml) for 30 min to block the synthesis of new S1P1 and then stimulated with 100 nM AKP-11 or FTY720 or FTY720P for 1 hr. The above compounds were washed out and replenished with fresh serum free medium containing 0.5% fatty acid free BSA and cycloheximide and incubated for 2 or 24 hrs. Data represents mean ± SEM of three independent experiments. Statistical significance is indicated as *<i>p</i><0.05 **<i>p</i><0.01 and ***<i>p</i><0.001, NS- not significant.