Knockdown of NS suppresses MHCC97H cell proliferation.

<p>(A) MTT assays of MHCC97H cells treated with siNC or siNS at 1, 2, and 3 days. Each value represents the average of three independent experiments, and bars indicate the standard error of the mean. *P<0.05, **P < 0.01. (B) Cell growth curves of MHCC97H cells were constructed by measuring cell proliferation every 12 h after transfection with siNS-and siNC for 24 h. (C and D) MTT assays of L02 cells treated with siNS or overexpressing NS. Each value represents the average of four independent experiments, and bars indicate the standard error of the mean. **P<0.01. (E) Knockdown of NS in MHCC97H cells by siRNA transfection at 48 and 72 h. Western blot analysis was performed using antibodies against NS and GAPDH as the loading control. (F)Western blotting assays of transient transfection of NS siRNA or overexpressed plasmids in L02 cells.