Enlarged bladders in <i>Mras</i>^<i>-/-</i> males.

<p>(A) Postmortem full bladder weights of male mice at different ages. Number of bladders tested: WT males: n = 15, three months; n = 17, six months; n = 21, 12 months. <i>Mras</i>^<i>-/-</i> males: n = 13, three months; n = 11, six months; n = 12, 12 months. Two-way ANOVA: There was a significant effect of genotype, F_1,83 = 39.93, <i>p</i><0.0001. However, a significant interaction between genotype and age was detected (<i>p</i> = 0.0142). The differences in bladder weights were significant at six months (<i>p</i> = 0.0104) and at 12 months (<i>p</i><0.0001). (B) Postmortem full bladder weights of female mice at three and six months of age. WT females: n = 8, three months; n = 7, six months. <i>Mras</i>^<i>-/-</i> females: n = 9 for both three and six months. Two-way ANOVA: not significant. (C) Bladder volumes of live males were determined by MRI; t-test: <i>p</i> = 0.0146; WT+hets, n = 7; <i>Mras</i>^<i>-/-</i>, n = 10. Error bars in (A, B, C) represent SEM.