Effect of culture components on CFA/I surface expression, LT secretion, and growth.

<p>The effect of individual components on CFA/I surface expression (A), LT secretion (B) and bacterial growth based on final density after overnight culture (C) was determined. The CFA/I expressing strain 258909–3 was cultured overnight in M9-low media supplemented with component combinations defined in the Hadamard matrix. CFA/I surface expression was evaluated by MRHA, LT toxin secretion by ELISA-GM1 of culture supernatant, and growth by OD₆₀₀. Components with a significant effect on a given response are shown in grey and those with no effect in black, with dashed lines representing the threshold of significance for each endpoint (p<5%). Coefficients are indicated for each component and indicate the relative strength of the response. PGM: porcine gastric mucin; 1,10 o-phen: 1,10-phenanthroline.