Regulatory T cells characterization by flow cytometry.

<p><b>A</b>, Representative staining of CD25⁺ FoxP3⁺ cells gated on CD4⁺ lymphocytes (T cells) in the lamina propria by flow cytometry. B, Representative staining of IL-10⁺ FoxP3⁺ and TGF-β⁺ FoxP3⁺ cells gated on CD4⁺CD25⁺ lymphocytes (anti-CD4 PE-Cy5, and anti-CD25 FITC or PE for membrane staining, and anti-FoxP3 PE or APC, anti-IL-10 APC and TGF-β APC for intra-cytoplasmic staining, isotype controls were included). Frequencies of cells are expressed as the mean values ±SEM. These results are representative of two independent experiments with similar results. Two-way ANOVA was used because all the data had normal distribution and equal variances. <i>***P</i><0.001, <i>**P</i><0.01, <i>*P</i><0.05.