Phenotype of the <i>L</i>. <i>japonicus scarn-1</i> mutant.

<p><b>(A)</b> Whole plants of wild-type Gifu B-129 and <i>scarn-1</i> five weeks after germination and growth in a 1:1 mix of perlite and vermiculite inoculated with <i>M</i>. <i>loti</i> R7A. Left: wild-type Gifu B-129; right: SL2564-3 (<i>scarn-1)</i>. <b>(B-C)</b> Nodules formed 3 weeks after <i>M</i>. <i>loti</i> inoculation of (B) wild type or (C) SL2564-3 (<i>scarn-1)</i>. <b>(D)</b> A nodule formed on <i>scarn-1</i> 5 weeks after inoculation. Panels E, F and G show light microscopy sections of the nodules shown in B, C and D respectively. Arrow in (G) shows the pockets of intercellular bacteria. <b>(H)</b> Nodule numbers 4 and 5 weeks after inoculation of wildtype and SL2564-3 <i>(scarn-1)</i> plants inoculated with <i>M</i>. <i>loti</i> R7A (the bar indicated 95% confidence intervals): grey columns: mature, pink nodules; white columns: white immature nodule bumps. Scale bars: A, 2 cm; B-D, 1 mm; and E, 100 μm; F-G, 200μm.