Effect of AmpliconDuo filtering on chimeras for prokaryotic sample Pro2.

<p>Chimeras defined by being recognized by UCHIME in <i>de novo</i> mode with score ≥ 1. Top: Frequency of chimeras in branches A, B of split sample as function of their read numbers, before (dashed lines) and after (solid lines) application of AmpliconDuo filter. Bottom: Fraction of chimeras passing the AmpliconDuo filter (<i>f</i>_{<i>filtered</i>}/<i>f</i>_{<i>unfiltered</i>}) for read numbers 1 to 20 in both branches A, B, and corresponding prediction <i>P</i>(<i>r</i>_<i>iA</i>, <i>r</i>_<i>iB</i> ≥ 1) using the Poisson model in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0141590#pone.0141590.e026" target="_blank">Eq (8)</a> with <i>λ</i>_<i>i</i> = 1, 2, …, 20.