Additional file 4: Figure S4. of Deletion of aquaporin-4 in APP/PS1 mice exacerbates brain Aβ accumulation and memory deficits

Genotyping of mice. An example of PCR analysis of AQP4 (the upper panel) and APPSwe (the low panel) mRNA expression in a newborn litter that is generated by AQP4+/−APP/PS1+/+ and AQP4+/−APP/PS1−/− mice. We only detected APPSwe transgene, because APPSwe transgene and PS1ΔE9 transgene are coexpressed under the control of the mouse prion promoter (Jankowsky et al. [60]). APP/PS1 transgene allele yields a 377-bp product; and wild type allele has no product. AQP4 knockout homozygote allele yields a 320-bp product; heterozygote allele yields 240-bp and 320-bp products; and wild type allele yields a 240-bp product. In this litter, eleven mice belong to the following 6 genotypes: AQP4+/+APP/PS1+/+ (mice 1 and 4), AQP4+/−APP/PS1−/− (mouse 2), AQP4+/−APP/PS1+/+ (mice 3, 8 and 9), AQP4−/−APP/PS1+/+ (mice 5 and 6), AQP4−/−APP/PS1−/− (mice 7 and 10), and AQP4+/+APP/PS1−/− (mouse 11). (TIF 2043 kb