SomA and Flo8 activate <i>FLO11</i> expression and act through similar regions of the <i>FLO11</i> promoter.

<p>(A) Expression of <i>FLO11</i>::<i>LacZ</i> was determined in haploid Y16870 strain expressing the indicated proteins or empty vector as negative control. (B) Schematic overview of 14 different 400 bp constructs of the <i>FLO11</i> promoter region fused to <i>CYC1</i>::<i>LacZ</i> reporter [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005205#ppat.1005205.ref031" target="_blank">31</a>]. (C) Expression of <i>LacZ</i> gene fused to different <i>FLO11</i> promoter fragments in Y16870 strain expressing the indicated proteins. In all experiments, strains were grown on 10 mL SC-Ura-Leu medium as pre-culture, then 1 mL of samples were inoculated into SC-Ura-Leu-Met medium as main culture for 6 h before the β-galactosidase activities were determined. Graph indicates mean ± standard error of triplicate measurements.