Characteristics of predicted multiple-point mutants of DhaA.

Location of stabilizing mutations in designed enzymes.

David Bednar (821277)
Koen Beerens (821278)
Eva Sebestova (821279)
Jaroslav Bendl (509922)
Sagar Khare (821280)
Radka Chaloupkova (492929)
Zbynek Prokop (821281)
Jan Brezovsky (126090)
David Baker (11642)
Jiri Damborsky (1322766)

November 2015

A) Locations of substitutions in energy-based, evolution-based and combined mutants of DhaA enzym...

Summary of molecular defects tested in the H2A mutants.

Christine E. Cucinotta (779688)
Alexandria N. Young (779689)
Kristin M. Klucevsek (779690)
Karen M. Arndt (779691)

August 2015

Phenotypes (listed on the left) of the histone mutants rated relative to wild type (WT) and addit...

Classification of mutants on the basis of their “in vitro” altered properties and the associated clinical phenotypes.

Laurent R. Chiarelli (237358)
Simone M. Morera (333198)
Paola Bianchi (333199)
Elisa Fermo (333200)
Alberto Zanella (333201)
Alessandro Galizzi (333202)
Giovanna Valentini (237355)

February 2013

a++: highly conserved; + conserved in vertebrates; − not conserved.baccording to Palmai et...

Characteristics of predicted multiple-point mutants of LinA.

David Bednar (821277)
Koen Beerens (821278)
Eva Sebestova (821279)
Jaroslav Bendl (509922)
Sagar Khare (821280)
Radka Chaloupkova (492929)
Zbynek Prokop (821281)
Jan Brezovsky (126090)
David Baker (11642)
Jiri Damborsky (1322766)

November 2015

a not applicableb activity determined with γ-hexachlorocyclohexane a...

Biochemical properties of DhaA wild-type and the final mutant DhaA115.

David Bednar (821277)
Koen Beerens (821278)
Eva Sebestova (821279)
Jaroslav Bendl (509922)
Sagar Khare (821280)
Radka Chaloupkova (492929)
Zbynek Prokop (821281)
Jan Brezovsky (126090)
David Baker (11642)
Jiri Damborsky (1322766)

November 2015

A) Melting temperatures of DhaA wild-type (blue) and DhaA115 (red) in the presence of indicated s...

Binding of docosahexaenoic acid (DHA) with cNLRX1 wildtype and mutant.

Pinyi Lu (120070)
Raquel Hontecillas (120113)
Vida Abedi (221302)
Shiv Kale (841480)
Andrew Leber (778578)
Chase Heltzel (841481)
Mark Langowski (841482)
Victoria Godfrey (841483)
Casandra Philipson (778579)
Nuria Tubau-Juni (841484)
Adria Carbo (120066)
Stephen Girardin (841485)
Aykut Uren (142901)
Josep Bassaganya-Riera (120051)

January 2016

A) Chemical structure of DHA. B) Interactions between DHA and cNLRX1 predicted by molecular docki...

Steady-state kinetic constants of DhaA wild-type and the final mutant Dha115 determined with 1-iodohexane at 37°C and 57°C, respectively, and pH 8.6.

David Bednar (821277)
Koen Beerens (821278)
Eva Sebestova (821279)
Jaroslav Bendl (509922)
Sagar Khare (821280)
Radka Chaloupkova (492929)
Zbynek Prokop (821281)
Jan Brezovsky (126090)
David Baker (11642)
Jiri Damborsky (1322766)

November 2015

K0.5 –concentration of substrate at half maximal velocity, kcat</su...

Kinetic parameters of OPH mutants in the DFP hydrolysis with or without triethanolamine (TEA).

Dandan Li (391591)
Yunze Zhang (6534)
Haitao Song (3639631)
Liangqiu Lu (3639628)
Deli Liu (694079)
Yongze Yuan (694074)

January 2017

Kinetic parameters of OPH mutants in the DFP hydrolysis with or without triethanolamine (TEA).</p

Thermal stability of FHbp ID 1 mutants measured by differential scanning calorimetry.

Monica Konar (752131)
Raffaella Rossi (208574)
Helen Walter (752132)
Rolando Pajon (166045)
Peter T. Beernink (166041)

June 2015

A. Wild-type (WT) FHbp ID 1 (dashed grey line) and Q38R mutant (solid black line). B-E....

FireProt : energy- and evolution-based computational design of thermostable multiple-point mutants

Bednar, David
Beerens, Koen
Sebestova, Eva
Bendl, Jaroslav
Khare, Sagar
Chaloupkova, Radka
Prokop, Zbynek
Brezovsky, Jan
Baker, David
Damborsky, Jiri

January 2015

There is great interest in increasing proteins' stability to enhance their utility as biocatalysts, ...

Iterative saturation mutagenesis of BbPK for dihydroxyacetone (DHA).

Yiqun Yang (1781026)
Yuwan Liu (9611925)
Haodong Zhao (6713204)
Dingyu Liu (2831702)
Jie Zhang (64655)
Jian Cheng (48763)
Qiaoyu Yang (715852)
Huanyu Chu (17020986)
Xiaoyun Lu (1390002)
Mengting Luo (6163199)
Xiang Sheng (1778470)
Yi-Heng P. J. Zhang (17020989)
Huifeng Jiang (245232)
Yanhe Ma (28802)

September 2023

The x-axis labels represent the location combinations in the BbPK. The y-axis labels represent the r...

The active residue sites of DLDH744.

Lingfeng Zhu (799124)
Xiaoling Xu (17877)
Limin Wang (87511)
Hui Dong (153701)
Bo Yu (241082)

September 2015

(a) The activities of mutant lactate dehydrogenases. (b) The activities of glutamate dehydrogenas...

Double mutant cycle analysis for His33 and Ser345.

Xin Liang (442470)
Huijuan Xu (442471)
Caiyue Li (442472)
Shikui Yin (442473)
Tingting Xu (307597)
Jinsong Liu (6516)
Zhiyuan Li (205620)

August 2013

(A) Mutant cycle analysis shows free energy changes between H33C and S345C. (B) Mutant cycle anal...

Features of native recombinant HsDH protein and its five clinically interesting patient mutation variants.

Maija L. Mehtälä (105023)
Marc F. Lensink (105027)
Laura P. Pietikäinen (105031)
J. Kalervo Hiltunen (105034)
Tuomo Glumoff (105038)

January 2013

The activities of human dehydrogenase and its variants were measured by detecting spectrophotomet...

Summary of the biophysical parameters of PfMDH mutants, as assessed by Static Light Scattering assay (SLS), Microscale Thermophoresis assay (MST) and Thermal Shift Assay (TSA).

Sergey Lunev (5142905)
Sabine Butzloff (5142896)
Atilio R. Romero (5142899)
Marleen Linzke (5142902)
Fernando A. Batista (5142893)
Kamila A. Meissner (5142908)
Ingrid B. Müller (43367)
Alaa Adawy (1609960)
Carsten Wrenger (37474)
Matthew R. Groves (43369)

April 2018

Summary of the biophysical parameters of PfMDH mutants, as assessed by Static Light Scatte...

Location of stabilizing mutations in designed enzymes.

David Bednar (821277)
Koen Beerens (821278)
Eva Sebestova (821279)
Jaroslav Bendl (509922)
Sagar Khare (821280)
Radka Chaloupkova (492929)
Zbynek Prokop (821281)
Jan Brezovsky (126090)
David Baker (11642)
Jiri Damborsky (1322766)

November 2015

A) Locations of substitutions in energy-based, evolution-based and combined mutants of DhaA enzym...

Summary of molecular defects tested in the H2A mutants.

Christine E. Cucinotta (779688)
Alexandria N. Young (779689)
Kristin M. Klucevsek (779690)
Karen M. Arndt (779691)

August 2015

Phenotypes (listed on the left) of the histone mutants rated relative to wild type (WT) and addit...

Classification of mutants on the basis of their “in vitro” altered properties and the associated clinical phenotypes.

Laurent R. Chiarelli (237358)
Simone M. Morera (333198)
Paola Bianchi (333199)
Elisa Fermo (333200)
Alberto Zanella (333201)
Alessandro Galizzi (333202)
Giovanna Valentini (237355)

February 2013

a++: highly conserved; + conserved in vertebrates; − not conserved.baccording to Palmai et...

Characteristics of predicted multiple-point mutants of LinA.

David Bednar (821277)
Koen Beerens (821278)
Eva Sebestova (821279)
Jaroslav Bendl (509922)
Sagar Khare (821280)
Radka Chaloupkova (492929)
Zbynek Prokop (821281)
Jan Brezovsky (126090)
David Baker (11642)
Jiri Damborsky (1322766)

November 2015

a not applicableb activity determined with γ-hexachlorocyclohexane a...

Biochemical properties of DhaA wild-type and the final mutant DhaA115.

David Bednar (821277)
Koen Beerens (821278)
Eva Sebestova (821279)
Jaroslav Bendl (509922)
Sagar Khare (821280)
Radka Chaloupkova (492929)
Zbynek Prokop (821281)
Jan Brezovsky (126090)
David Baker (11642)
Jiri Damborsky (1322766)

November 2015

A) Melting temperatures of DhaA wild-type (blue) and DhaA115 (red) in the presence of indicated s...

Binding of docosahexaenoic acid (DHA) with cNLRX1 wildtype and mutant.

Pinyi Lu (120070)
Raquel Hontecillas (120113)
Vida Abedi (221302)
Shiv Kale (841480)
Andrew Leber (778578)
Chase Heltzel (841481)
Mark Langowski (841482)
Victoria Godfrey (841483)
Casandra Philipson (778579)
Nuria Tubau-Juni (841484)
Adria Carbo (120066)
Stephen Girardin (841485)
Aykut Uren (142901)
Josep Bassaganya-Riera (120051)

January 2016

A) Chemical structure of DHA. B) Interactions between DHA and cNLRX1 predicted by molecular docki...

Steady-state kinetic constants of DhaA wild-type and the final mutant Dha115 determined with 1-iodohexane at 37°C and 57°C, respectively, and pH 8.6.

David Bednar (821277)
Koen Beerens (821278)
Eva Sebestova (821279)
Jaroslav Bendl (509922)
Sagar Khare (821280)
Radka Chaloupkova (492929)
Zbynek Prokop (821281)
Jan Brezovsky (126090)
David Baker (11642)
Jiri Damborsky (1322766)

November 2015

K0.5 –concentration of substrate at half maximal velocity, kcat</su...

Kinetic parameters of OPH mutants in the DFP hydrolysis with or without triethanolamine (TEA).

Dandan Li (391591)
Yunze Zhang (6534)
Haitao Song (3639631)
Liangqiu Lu (3639628)
Deli Liu (694079)
Yongze Yuan (694074)

January 2017

Kinetic parameters of OPH mutants in the DFP hydrolysis with or without triethanolamine (TEA).</p

Thermal stability of FHbp ID 1 mutants measured by differential scanning calorimetry.

Monica Konar (752131)
Raffaella Rossi (208574)
Helen Walter (752132)
Rolando Pajon (166045)
Peter T. Beernink (166041)

June 2015

A. Wild-type (WT) FHbp ID 1 (dashed grey line) and Q38R mutant (solid black line). B-E....

FireProt : energy- and evolution-based computational design of thermostable multiple-point mutants

Bednar, David
Beerens, Koen
Sebestova, Eva
Bendl, Jaroslav
Khare, Sagar
Chaloupkova, Radka
Prokop, Zbynek
Brezovsky, Jan
Baker, David
Damborsky, Jiri

January 2015

There is great interest in increasing proteins' stability to enhance their utility as biocatalysts, ...

Iterative saturation mutagenesis of BbPK for dihydroxyacetone (DHA).

Yiqun Yang (1781026)
Yuwan Liu (9611925)
Haodong Zhao (6713204)
Dingyu Liu (2831702)
Jie Zhang (64655)
Jian Cheng (48763)
Qiaoyu Yang (715852)
Huanyu Chu (17020986)
Xiaoyun Lu (1390002)
Mengting Luo (6163199)
Xiang Sheng (1778470)
Yi-Heng P. J. Zhang (17020989)
Huifeng Jiang (245232)
Yanhe Ma (28802)

September 2023

The x-axis labels represent the location combinations in the BbPK. The y-axis labels represent the r...

The active residue sites of DLDH744.

Lingfeng Zhu (799124)
Xiaoling Xu (17877)
Limin Wang (87511)
Hui Dong (153701)
Bo Yu (241082)

September 2015

(a) The activities of mutant lactate dehydrogenases. (b) The activities of glutamate dehydrogenas...

Double mutant cycle analysis for His33 and Ser345.

Xin Liang (442470)
Huijuan Xu (442471)
Caiyue Li (442472)
Shikui Yin (442473)
Tingting Xu (307597)
Jinsong Liu (6516)
Zhiyuan Li (205620)

August 2013

(A) Mutant cycle analysis shows free energy changes between H33C and S345C. (B) Mutant cycle anal...

Features of native recombinant HsDH protein and its five clinically interesting patient mutation variants.

Maija L. Mehtälä (105023)
Marc F. Lensink (105027)
Laura P. Pietikäinen (105031)
J. Kalervo Hiltunen (105034)
Tuomo Glumoff (105038)

January 2013

The activities of human dehydrogenase and its variants were measured by detecting spectrophotomet...

Summary of the biophysical parameters of PfMDH mutants, as assessed by Static Light Scattering assay (SLS), Microscale Thermophoresis assay (MST) and Thermal Shift Assay (TSA).

Sergey Lunev (5142905)
Sabine Butzloff (5142896)
Atilio R. Romero (5142899)
Marleen Linzke (5142902)
Fernando A. Batista (5142893)
Kamila A. Meissner (5142908)
Ingrid B. Müller (43367)
Alaa Adawy (1609960)
Carsten Wrenger (37474)
Matthew R. Groves (43369)

April 2018

Summary of the biophysical parameters of PfMDH mutants, as assessed by Static Light Scatte...

Location of stabilizing mutations in designed enzymes.

David Bednar (821277)
Koen Beerens (821278)
Eva Sebestova (821279)
Jaroslav Bendl (509922)
Sagar Khare (821280)
Radka Chaloupkova (492929)
Zbynek Prokop (821281)
Jan Brezovsky (126090)
David Baker (11642)
Jiri Damborsky (1322766)

November 2015

A) Locations of substitutions in energy-based, evolution-based and combined mutants of DhaA enzym...

Summary of molecular defects tested in the H2A mutants.

Christine E. Cucinotta (779688)
Alexandria N. Young (779689)
Kristin M. Klucevsek (779690)
Karen M. Arndt (779691)

August 2015

Phenotypes (listed on the left) of the histone mutants rated relative to wild type (WT) and addit...

Classification of mutants on the basis of their “in vitro” altered properties and the associated clinical phenotypes.

Laurent R. Chiarelli (237358)
Simone M. Morera (333198)
Paola Bianchi (333199)
Elisa Fermo (333200)
Alberto Zanella (333201)
Alessandro Galizzi (333202)
Giovanna Valentini (237355)

February 2013

a++: highly conserved; + conserved in vertebrates; − not conserved.baccording to Palmai et...

Characteristics of predicted multiple-point mutants of DhaA.

Abstract

Extracted data

Characteristics of predicted multiple-point mutants of DhaA.

Abstract

Extracted data

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