Relative expression of the <i>myod</i> and <i>myogenin</i> mRNAs in pacu myoblast cell cultures.

<p><i>myod</i> and <i>myogenin</i> mRNAs expression was assessed by qPCR in primary myoblast cultures isolated from fast-twitch muscle of juvenile pacus at different phases: 2–4 days (2–4 d), 5–8 days (5–8 d) and 9–12 days (9–12 d). The data are expressed as the fold change compared with the expression level at 2–4 days. The data are presented as the mean ± SEM from 3 independent cell cultures. The different letters indicate significant differences in expression (p<0.05).