Effect of methyl sulfone on metastatic induction of miR-210-3p and miR-210-5p.

<p>(A) 66cl-4 metastatic breast cancer cells were grown to confluence in the presence and absence of methyl sulfone. At confluence, half of the control plates (no methyl sulfone) and half of the methyl sulfone-treated plates were placed under hypoxia at 37°C. The remaining plates continued incubation under normoxic conditions. After six hours cells were solubilized in TRIzol to isolate RNA. Relative levels of miRNA-210-3p and miR-210-5p were determined by microarray analysis and real time QPCR, in triplicate. Six pairwise comparisons of the four samples for each miR are shown (A) along with Log2 differences between pairs. (B) Relative levels of individual miR-210 were determined.