FL3 rapidly induces phosphorylation of STAT3.

<p><b>A.</b> STAT3 coimmunoprecipitates (co-IP) with PHB1. An anti-FLAG antibody was incubated with extracts of the H9c2 cardiomyocytes. Immunoprecipitates were resolved by means of SDS-PAGE and probed for STAT3 and the FLAG tag to detect both PHB1 and PHB2. <b>B.</b> Representative western blots of protein lysates of H9c2 cells treated with FL3 (100 nM), by means of antibodies that recognize either phosphorylated (Tyr⁷⁰⁵) or total STAT3 protein. <b>C.</b> Quantitative analysis of the western blots (percentage of phosphorylated STAT3 in total STAT3, <i>n</i> = 4; *p < 0.05, compared to control; **p < 0.001, compared to control; ***p < 0.01, compared to control). <b>D</b> and <b>E.</b> Effects of the Janus kinase 2 (JAK2) inhibitor WP1066 on STAT3 phosphorylation: Representative western blots and quantitative analysis (percentage of phosphorylated STAT3 in total STAT3, <i>n</i> = 4; p < 0.05, compared to control; **p < 0.05, compared to FL3).