Quantification of birefringence intensity in normal siblings (<i>Sib</i>, open bars) and mutated larvae of <i>Sapje</i> (<i>Sap</i>, filled bars) and <i>Candyfloss</i> (<i>Caf</i>, hatched bars), at 4 dpf.

<p>Larvae were treated with 0.1% DMSO or 50 μM BTS. n = 3–4 in each group. **, p< 0.01 *** p< 0.001 compared to the <i>Sib</i> in DMSO, ###, p<0.001 compared to the mutants (<i>Caf</i> or <i>Sap</i>) in DMSO (ANOVA, Holm-Sidak method). No significant difference was detected between the BTS treated groups.