Mouse <i>Tctn1</i> genetically interacts with <i>Bbs1</i>.

<p>(A) Lateral views of wild type, <i>Tctn1</i>^-/-, <i>Bbs1</i>^-/- and <i>Tctn1</i>^-/-<i>Bbs1</i>^-/- embryos at E14.5. Exencephaly is apparent in the <i>Tctn1</i>^-/-<i>Bbs1</i>^-/- double mutant. Alcian blue staining of the corresponding genotype’s forelimb (top) and hindlimb (bottom) are included, with asterisks indicating extra digits. Scale bars, 1 mm. (B-D) Number of digits in the (B) forelimbs and (C) hindlimbs, and (D) the incidence of exencephaly for the indicated genotypes. Numbers of animals analyzed for polydactyly and exencephaly are shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005627#pgen.1005627.s007" target="_blank">S3</a> and <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005627#pgen.1005627.s008" target="_blank">S4</a> Tables. (E) Limb bud fibroblasts of the indicated genotypes immunostained for Tub^Ac (green), Arl13b (red), γ-tubulin (cyan), and DAPI (blue). Scale bar, 10 μm. (F) Quantification of proportion of ciliated limb bud fibroblasts. Error bars represent the standard deviation. Statistical significance according to unpaired Student’s <i>t</i>-tests (* <i>p</i><0.0001). (G) Limb bud fibroblasts immunostained for Nphp1 (red), Tub^Ac (green), and γ-tubulin (blue). Scale bar, 1 μm.