Mouse <i>Tctn1</i> genetically interacts with NPHP complex genes, but not MKS complex genes.

<p>(A) Lateral views of wild type, single or double mutant mouse embryos of indicated genotype at E14.5. Exencephaly is apparent in the <i>Tctn1</i>^-/-<i>Nphp1</i>^-/- double mutant. Corresponding Alcian blue staining of the right forelimb (top) and hindlimb (bottom) are included, with asterisks indicating extra digits. Genes encoding components of the NPHP complex are indicated in red. Genes encoding components of the MKS complex are indicated in green. Scale bars, 1 mm. (B) Number of digits in the forelimbs and (C) hindlimbs of wild type, single or double mutant embryos of indicated genotypes. (D) Incidence of exencephaly in wild type, single or double mutants embryos of indicated genotypes. Numbers of animals analyzed for polydactyly and exencephaly are included in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005627#pgen.1005627.s005" target="_blank">S1</a> and <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005627#pgen.1005627.s006" target="_blank">S2</a> Tables.