TCTN-1 contributes to <i>C</i>. <i>elegans</i> ciliary gate function.

<p>(A-D) Localization of different transition zone proteins in phasmid cilia of wild type, <i>tctn-1</i>, <i>nphp-4</i>, and <i>tctn-1; nphp-4</i> mutants. Each GFP, CFP, YFP, or tdTomato-tagged transition zone protein is co-localized with a ciliary marker (CHE-13, DYF-11 or XBX-1), as indicated. Abnormal localization of MKS-5 in the <i>nphp-4</i> single and <i>tctn-1; nphp-4</i> double mutants is indicated by asterisks. (E) ARL-13::GFP localizes ectopically to the PCMC in <i>tctn-1</i>, <i>nphp-4</i>, and <i>tctn-1; nphp-4</i> mutants as indicated by asterisks, but localizes normally to the middle segment (MS) of cilia. (F) ODR-10::GFP localizes to the cilia of AWA neurons in wild type, <i>tctn-1</i>, <i>nphp-4</i>, and <i>tctn-1; nphp-4</i> mutants. Anterior is to the right. (G) TRAM-1a::tdTomato localizes only to the PCMC in wild type animals, but enters phasmid cilia in <i>tctn-1</i> mutants, as indicated by asterisks. MKS-2::GFP marks the transition zone. Scale bars, 5 μm.