Endocrine differentiation of pancreas is impaired in <i>Pdx1</i>^<i>tTA/+</i><i>;tetO</i>^<i>MafA</i> pancreas.

<p>At E17.5 Isl1 (green, <b>AB</b>), Pax6 (green, <b>CD</b>) and Hb9 (green, <b>EF</b>), transcription factors implicated in endocrine differentiation and maturation, have severely reduced expression in <i>Pdx1</i>^<i>tTA/+</i><i>;tetO</i>^<i>MafA</i> pancreas (<b>ACE</b>) compared to <i>tetO</i>^<i>MafA</i> pancreas (<b>BDF</b>). This finding is consistent with their reduced number of insulin⁺ cells (red, <b>A-F</b>), and suggests that misexpression of the MafA transgene inhibits the entire endocrine differentiation program. DAPI (blue). Bar: 50 μm.