<i>UFBP1</i> deficiency impairs lineage development of erythroid progenitors.

<p><b>a</b>. Flow cytometry analysis of erythroid and myeloid lineages in control and <i>UFBP1</i> deficient mice after TAM injection. BM cells were collected from TAM-injected control (n = 4) and floxed (n = 4) male mice when <i>UFBP1</i> deficient mice exhibited significant weight loss and became moribund. The following markers were used for flow analysis: BV510-Sca-1, APC780-c-Kit, PE-Cy7-CD150, Alexa 700-CD16/32, PE-IL-7R, APC-CD41, BV650-CD105, FITC-CD71, BV421-TER119, and lineage markers including PerCP-Cy5.5-conjugated CD4, CD8, CD3, CD5, Gr-1, CD11b, CD19 and B220. <b>b.</b> The percentages of each lineage in L^-S^-K⁺ cells. *p <0.01, and **p <0.05 (n = 4). MkP: megakaryocyte progenitor; GMP: granulocyte macrophage progenitor; Pre GM: granulocyte macrophage precursor; Pre MegE: megakaryocyte erythroid precursor; Pre CFU-E: CFU-E precursor; CFU-E: erythroid colony-forming unit; Pro Ery: proerythroblast. <b>c</b>. The absolute cell numbers of each lineage in control and UFBP1-depleted BMs. UFBP1 depletion led to reduction of total BM cells (1 x 10⁸ cells in control versus 5 x 10⁷ in <i>UFBP1</i> deficient mice). *p <0.01 (n = 4).