Loss of ATMIN in combination with NBS1, in T cells, leads to increased mortality due to T cell activation.

<p>(A) Kaplan-Meier survival curve of control, ATMIN^ΔL, NBS1^ΔL, ATMIN^ΔLNBS1^ΔL and ATM^-/- mice. Survival was monitored for 32 weeks. (B) Representative images of spleens of control, ATMIN^ΔL, NBS1^ΔL and ATMIN^ΔLNBS1^ΔL mice as well as a moribund ATMIN^ΔLNBS1^ΔL mouse. (C) Histological analysis of the spleen of a control and a moribund ATMIN^ΔLNBS1^ΔL mouse stained for T cells using an anti-CD3 antibody. (D) Representative flow cytometry data of CD4 and CD8 T cells (gated on the TCRβ⁺ population) in the spleen of control, ATMIN^ΔL, NBS1^ΔL and ATMIN^ΔLNBS1^ΔL mice. (E) Histological analysis by using an anti-CD3 antibody to visualize T cells in the liver and lung of control and ATMIN^ΔLNBS1^ΔL mice. (F) Representative flow cytometry data of activated CD62L^lowCD44⁺ T cells (gated on the TCRβ⁺ population) in the spleen of control, ATMIN^ΔL, NBS1^ΔL and ATMIN^ΔLNBS1^ΔL mice. (G) Quantification of F. N = 3–4 mice per genotype. (H) Flow cytometry data showing the percentage of antigen-experienced CD62L^lowCD4⁺ T cells (gated on the TCRβ⁺ population) in the spleen of control and ATMIN^ΔLNBS1^ΔL mice as well as moribund ATMIN^ΔLNBS1^ΔL mice. (I) Representative flow cytometry data of proliferating (BrdU⁺) T cells (gated on the TCRβ⁺ population) in the spleen of mice indicated in F, measured by <i>in vivo</i> BrdU incorporation over a period of 4 days. (J) Quantification of I. N = 3–4 mice per genotype. Error bars represent SEM (*<i>P</i><0.05, ***<i>P</i><0.001).