ATMIN^ΔLNBS1^ΔL mice display enhanced cytokine and ROS production.

<p>(A) Gene ontology analysis of processes enriched in the spleen of ATMIN^ΔLNBS1^ΔL mice identified by mRNA-sequencing. (B) Quantitative RT-PCR analysis of selected genes found to be enriched in A in the spleens of control, ATMIN^ΔL, NBS1^ΔL and ATMIN^ΔLNBS1^ΔL mice. Gene expression is normalized to mef1α control. (C) Quantitative RT-PCR analysis of expression of proinflammatory cytokines Il17a, Tnfα, Il1β and Ifnγ in splenic T cells of indicated mice with or without 12 hours <i>in vitro</i> stimulation with anti-CD3 and anti-CD28 antibodies. Gene expression is normalized to mef1α control. N = 4–8 mice per genotype. (D) Representative intracellular expression of IL17A and IFNγ assessed by flow cytometry, in YFP^- and YFP⁺ T (gated on the CD4⁺ population) cells in the spleen of control and ATMIN^ΔLNBS1^ΔL mice after PMA and ionomycin stimulation. (E) Enrichment analysis of anti-oxidative genes in the spleen of ATMIN^ΔLNBS1^ΔL mice identified by mRNA-sequencing. (F) Quantitative RT-PCR analysis of selected genes found to be enriched in E in the spleens of control, ATMIN^ΔL, NBS1^ΔL and ATMIN^ΔLNBS1^ΔL mice. Gene expression is normalized to mef1α control. (G) Splenic cells from control, ATM^-/-, ATMIN^ΔL, NBS1^ΔL and ATMIN^ΔLNBS1^ΔL mice were stained with the CellROX reagent and counterstained with Hoechst 33258. Error bars represent SEM (*<i>P</i><0.05, **<i>P</i><0.01, ****<i>P</i><0.0001).