VCP K315 trimethylation is abolished in <i>Vcpkmt</i>^<i>-/-</i> mice tissues.

<p>(<b>A</b>) Mass spectrometric analysis of VCP isolated from wild-type (<i>Vcpkmt</i>^<i>+/+</i>) and <i>Vcpkmt</i>^<i>-/-</i> mouse tissues. Extracted ion chromatograms corresponding to un-, di- and trimethylated K315 in Arg-C-generated peptide VCP (314–322) are shown. For each tissue the intensity is normalized to the Kme3 signal. Expected elution time of Kme0 (left panel) or Kme3 (right panel) peptides are indicated with an arrow. The data for wild-type tissues have been published previously [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0141472#pone.0141472.ref006" target="_blank">6</a>]. (<b>B</b>) Immunoblotting for K315me3-VCP in various wild-type and <i>Vcpkmt</i>^<i>-/-</i> tissue protein extracts. 30 μg of whole cell extracts are loaded. Total VCP is shown as a loading control.