Multiple ovary phenotypes are observed following developmental silencing of <i>dsx</i>.

<p>Adult females that were injected with <i>dsx-KD A</i> or <i>dsx-KD B</i> siRNA as pupae had decreased ovary length both pre- (A) and post-blood meals (B). Representative ovaries from control-, <i>dsx-KD A</i>, or <i>dsx-KD B</i> stained with phalloidin (red) and the nuclear marker TO-PRO-3 (blue) are shown below each graph in A and B. In comparison to control microinjected females, the number of ovarioles/ovary (E) and length of each ovariole (F) post-blood meal was significantly less in females microinjected with <i>dsx-KD A</i> and <i>dsx-KD B</i> siRNA. Means for each control or experimental group are reported, and these were determined through the combination of data gathered in at least three biological replicate experiments. Error bars represent standard deviations. *** = P < 0.001.