Effect of brain hypoxia-ischemia on the production of reactive oxygen species in suspension of acutely isolated cells, measured using DCFH-DA.

<p><b>Percentage of DCFH-DA positive cells at different time points after hypoxia-ischemia</b>: (<b>A) 0 h, (B) 3 h and (C) 12 h. Relative fluorescence intensity of cells with <i>in vivo</i> marker DCFH-DA at different time points after hypoxia-ischemia</b>; (<b>D) 0 h, (E) 3 h and (F) 12 h</b>, in control (n≥5), HI (n≥5) and animals pretreated with resveratrol (n≥5) groups. Asterisks denote the significance levels when compared to the control group (<b>***</b><i>P</i><0.0001 <b>*</b><i>P</i><0.05). The hash symbols denote the significance levels when compared to the hypoxia-ischemia group (^###<i>P</i><0.0001 or ^#<i>P</i><0.05).