Transcriptional organization analysis of <i>phaZ</i>, <i>phaP</i>, <i>phaC2</i>, <i>phaJ</i> and <i>fadE</i> genes.

<p>Strain JMP134 was grown in MSM supplemented with 2% octanoate (w/v) at 30°C, and mRNA was extracted at the early stationary phase. The cDNA was synthesized with random primers. (A) Schematic of the gene cluster containing <i>phaC2</i>. The locations and number of nucleotides of DNA fragments amplified by PCR are represented by short solid lines below the relevant genes and designated RTZP, RTPC2, RTC2J and RTJE. (B) Electrophoresis of PCR products from transcriptional organization analysis of RTZP, RTPC2, RTC2J and RTJE. Lane M, molecular marker; +, presence of RT-PCR products; -, the corresponding negative controls with DNase-treated RNA samples.