Selective requirement for IRF4 in TLR stimulated DKO BMDCs.

<p>(<b>A</b>) WT, DKO and CD11c-Cre IRF4^fl/fl DKO BMDCs were generated <i>in vitro</i> in presence of GM-CSF for 7–9 days prior to FACS analysis of MHCII, CD86 and PDL2. Histograms show relative expression of the indicated marker on WT (red), DKO (blue) and CD11c-Cre IRF4^fl/fl DKO (green) total CD11c⁺DCs or GFP⁺CD11c⁺DCs. One representative experiment out of 3 independent experiments is shown. <b>(B-C)</b> BMDCs were generated <i>in vitro</i> for 7 days. CD11c⁺DCs were purified by magnetic sorting followed by in vitro stimulation with 0.1<b>μ</b>g/ml LPS or 3<b>μ</b>M CpG, for 24h. IL-10 and IFN<b>β</b> gene expression in LPS treated (<b>□</b>) or CpG treated (<b>C</b>) WT, DKO and CD11c-Cre IRF4^fl/fl DKO BMDCs were assayed by qPCR. One representative experiment out of 4 independent experiments is shown. *: p<0.05; **: p<0.01, ***: p<0.001.