Additional file 2: Figure S1. of Transplantation of rat embryonic stem cell-derived retinal progenitor cells preserves the retinal structure and function in rat retinal degeneration

Construction of Rax::EGFP reporter rESCs. a Knock-in strategy for the Rax::EGFP reporter construct. Red arrows, PCR primers for genotyping; b Genotyping of Rax::EGFP knock-in. (primer pair: Rax-FP/EGFP-RP); c Genotyping of Rax::EGFP knock-in. (primer pair: Rax-FP/Rax-RP); d Sequencing of wild type allele. (PCR product from Rax-FP/Rax-RP); e Sequencing of Rax::EGFP knock-in allele. (PCR product from Rax-FP/EGFP-RP). (TIFF 4455 kb