Islets from STAT4 deficient mice are resistant to PIC-induced apoptosis.

<p>(A) Fluorescence microscopy was used to image apoptotic islets labeled with YO-PRO-1 (green) and PI (red). Islets from WT mice (top row) or islets from STAT4ko mice (bottom row) were treated with PICs, IL-1β/TNF-α, IL-1β/IFN-γ, or TNF-α/IFN-γ. (B) Graph shows quantified apoptosis from cytokine treated STAT4ko islets. (C) Graph shows quantified apoptotic index from cytokine treated WT islets vs STAT4ko islets. * <i>p</i> < 0.05, ** <i>p</i> < 0.01, *** <i>p</i> < 0.001 relative to ctl, +++ <i>p</i> < 0.001, ### <i>p</i> < 0.001 relative to WT PIC treatment. Three groups of each condition were treated and analyzed.