Changes in cell morphology in <i>L</i>. <i>major</i> promastigotes upon treatment.

<p>Compound 1 and 2 induce parasite swelling and vacuolization of <i>L</i>. <i>major</i> promastigotes at very early time points. <i>L</i>. <i>major</i> promastigotes were treated with solvent control (1% DMSO), miltefosine (122.7 μM), compound 1 (100 μM), and 2 (100 μM) for 30 min, 1 h, 2 h, 4 h, and 24 h. Cytospin preparations of the cells were stained with Diff-Quik dye and analyzed by transmitted light microscopy under 50× objective. “Plus” symbol (+) was used to represent changes in morphology; two (++) and three (+++) symbols were used as a reference for the severity of the phenotype induced by the different tested compounds and the black arrows to indicate the presence of vacuole-like structures. Black bar indicates 20 μm.