Macrophage depletion did not improve renal pathology in Col4a3KO mice.

<p>Kidney pathology was comparable between KO+PBSL and KO+CL. Panels <b>A</b> (H&E) and <b>C</b> (PAS) show tubular pathology (atrophy, degeneration/regeneration and eosinophilic casts) in KO+PBSL; panels <b>B</b> (H&E) and <b>D</b> (PAS) show comparable tubular pathology in KO+CL. Incidence of sclerotic glomeruli was comparable between KO+PBSL and KO+CL (circle = sclerotic glomeruli; star = normal-appearing glomeruli), with many glomeruli showing variable extent of changes from normal to sclerotic appearance. (<b>E</b>) Semi quantitative histologic assessment of nephropathy and sclerotic glomeruli revealed no significant differences between KO, KO+PBSL or KO+CL mice. Welch corrected ANOVA followed by posthoc pairwise t-test with a Bonferroni correction was used to evaluate differences between groups. (E) n = 3–5 mice per group. Scale bar 100 um. CL: clodronate liposomes; PBSL: PBS liposomes.