Endogenous BDNF and TrkB levels in retina and SC at various time points after ONC.

<p>(A) In the retina, the total BDNF levels, analyzed via ELISA, rise upon ONC, reaching a peak at 3 to 5 d, attenuating afterwards. (B) The retinal TrkB-FL levels only slightly increase in the ONC model, while TrkB-T1 inclines more, reaching peak levels between 24 h and 5 d post-ONC. (C) In the SC, total BDNF concentrations are enhanced as soon as 6 h post-ONC and slowly decrease to baseline levels thereafter. (D) Collicular TrkB-FL and TrkB-T1 levels follow a very similar increase-decrease pattern after ONC induction, with the turning point situated around 3 d post-ONC. Key: BDNF, brain-derived neurotrophic factor; TrkB, tropomyosin receptor kinase B; TrkB-FL, TrkB full-length form; TrkB-T1, TrkB truncated form 1; SC, superior colliculus; ONC, optic nerve crush.