Additional file 2: Figure S2. of Reduced β-amyloid pathology in an APP transgenic mouse model of Alzheimer’s disease lacking functional B and T cells

Lack of amyloid plaque deposition at 3 months. (A) Thioflavin-S staining confirms absence of amyloid plaque deposition in 3 month-old PSAPP and age-matched PSAPP/Rag2 ko mice (representative images of cortical stainings are shown). (B) Quantitative MSD analysis of Aβ40 and Aβ42 in plasma and cortical tissue homogenized using sequential extraction to obtain different protein fractions: Tris soluble; SDS detergent soluble; formic acid soluble fraction (B). Statistical analysis reveals no differences in Aβ levels between PSAPP/Rag2 ko mice and the PSAPP mice at 3 months. Scale bar = 300 μm. n = 14–15 per group. (TIF 26755 kb