Effects of pimecrolimus treatment on the externalization of CD63 and CD164.

<p>The frequency [%] of CD63⁺CD164⁺ cells among the total basophils (gated as SSC^lowCD193⁺CD203c⁺) is shown. The IL-3-primed basophils were pre-treated or not with 2.5 μMol pimecrolimus and analyzed prior to (<b>A</b>) and after activation with anti-IgE (<b>B</b>), birch pollen allergen Bet v 1a at 100 ng*ml^-1 (<b>C</b>) and 10 ng*ml^-1 (<b>D</b>), and hymenopteran venom at 1.0 μg*ml^-1 (<b>E</b>) and 0.1 μg*ml^-1 (<b>F</b>). The Tukey box plots are shown, the mean values are presented as lines, and the 5^th and 95^th percentiles are displayed as symbols. The significance was examined by the paired t-tests with Bonferroni correction at n = 18 and is indicated as *** (p_corr<0.001 = p_uncorr<0.000056), ** (p_corr<0.01 = p_uncorr<0.00056), * (p_corr<0.05 = p_uncorr<0.0028) and n.s. (p_corr>0.05 = p_uncorr<0.0028).