Neurovirulence of MAD4 and selected recombinant viruses in PVR-Tg mice.

<p>PVR-Tg21 mice expressing the human PV receptor were inoculated intracerebrally with 10⁵ TCID50 of virus (six mice per virus). Animals were checked daily for 21 days post-inoculation for paralysis or death. The number of healthy mice following inoculation with group I/I recombinants (A) or group II/I recombinants (B) relative to that following inoculation with the parental cVDPV MAD4 is shown. Survival curves are numbered to indicate overlap. No additional mice suffered paralysis or died after day 15 post-inoculation. *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001 in Log Rank tests comparing each selected recombinant virus with MAD4 (<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005266#ppat.1005266.s017" target="_blank">S6 File</a>).