ELISA of VGF peptides in mouse.

<p><u>Upper panel</u> (plasma): upon fasting (0’), higher VGF C-terminus, NAPPE, and ERVW immunoreactive peptide/s were shown in slim <i>vs</i>. obese mice, while the glucose load (120’) led to increased VGF C-terminus and TLQP peptide/s in slim mouse, with little response in the obese group. <u>Middle panel</u> (brown adipose tissue, BAT): TLQP, NAPPE and ERVW peptides were higher during fasting (0’) in slim mice, which also showed a distinct increase of VGF C-terminus peptide/s after the glucose load (120’). <u>Lower panel</u> (white adipose tissue, WAT): in basal conditions (0’) ERVW peptide/s levels were higher in slim mice, while the glucose load (120’) resulted in a response of all other VGF peptides in slim mice (only). VGF C-term: VGF C-terminus peptide/s; ERVW refers to the C-terminally directed assay used for QQET-30 like peptide/s (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0142333#pone.0142333.t001" target="_blank">Table 1</a>); mean±SEM, * = P<0.03.