<i>Ogt</i> is at least partially permissive for proliferation and migration.

<p><b>A:</b> Representative image (magnification 10x) showing cell staining for a transwell migration assay performed after enhancing O-GlcNAc levels, and <b>B:</b> Quantification of the migrated cells demonstrates that increasing protein O-GlcNAcylation does not alter cell motility relative to untreated cells. Negative controls are also shown. <b>C:</b> Cell staining is shown (magnification 10x) for cells carrying floxed <i>Ogt</i> and the effect of gene deletion in a migration assay, quantified in <b>D:</b> demonstrating that loss-of-function for <i>Ogt</i> significantly reduces the number of cells that can migrate. This effect is also borne out in negative controls. Individual data points are plotted and the horizontal line represents the mean, n = 3/group, *p < 0.05 vs Veh/OGT FL.