Expression of TCF/LEF transcription factors appears reduced in LX-2 cells.

<p>(A) Western Blot for TCF1, TCF3, TCF4 and LEF1in separate samples of LX-2 and HEK293 cells (B) TOPFLASH assay in HEK293 cells after 24hours incubation with conditioned medium from either HEK293 or LX-2 cells, (n = 4). (C) TOPFLASH assay in LX-2 cells overexpressing LEF1 and Ser37-βcatenin (n = 3) (D) TOPFLASH assay in HEK293 cells overexpressing Ser37-βCatenin or Ser37-βCatenin and sFRP4, (n = 3). Luciferase results represented as fold change of Firefly to Renilla *p<0.05 (Student’s <i>t</i>-test).