HMGB1 knockdown in primary striatal neurons without 3-NP treatment.

<p>(A,B) Primary striatal neurons were transduced with lentiviral vectors expressing either non-targeted control shRNA (Con-shRNA) or one of two different shRNAs targeting HMGB1 expression (HMGB1-shRNA1 and HMGB1-shRNA2) and cultured for 48 h. Total cellular extracts were subjected to Western blotting for HMGB1, LC3, SQSTM1, p-JNK, and JNK expression analysis. Densities of protein bands were analyzed with an image analyzer (Sigma Scan Pro 5) and normalized to the loading control (β-actin). Bars represent mean ± SE; n = 3 samples per group. Groups were compared by ANOVA followed by Dunnet’s post hoc test before data conversion. **p <0.01 vs control shRNA.