Tc-SMP proteins secreted into the extracellular medium by epimastigotes and metacyclic trypomastigotes (clone Dm28c) identified by proteomic analysis.

<p>⁽¹⁾ TriTrypDB entries are prefixed by the species name they were derived: TvY486_ for T. vivax; and TcCLB and TCDM_ for T. cruzi clone CL Brener and Dm28c, respectively.</p><p>^{(2 and 3)} To validate the quality of protein identification, the following parameters were used: Xcorr (CrossCorr / avg [AutoCorr offset = -75 to 75]) ≥ 1.5, 2.0, and 2.5, for singly, doubly and triply charged peptides, respectively. DC<i>n</i> (Xcorr₁ –Xcorr₂ / Xcorr1) ≥ 0.1.</p><p>⁽⁴⁾ Sample enriched in plasma membrane-derived vesicles or ectosomes (V16) from epimastigotes (Epi) or metacyclic trypomastigotes (Meta).</p><p>⁽⁵⁾ Sample enriched in soluble proteins (vesicle-free, VF) from epimastigotes (Epi).</p><p>Tc-SMP proteins secreted into the extracellular medium by epimastigotes and metacyclic trypomastigotes (clone Dm28c) identified by proteomic analysis.