Effect of ethanolamine and EutR on SPI-1.

<p>(<b>A</b>) qRT-PCR of <i>sipC</i> from WT <i>S</i>. Typhimurium (SL1344) grown in LB or LB supplemented with 5 mM ethanolamine (EA). (<b>B</b>) qRT-PCR of <i>sipC</i> from WT <i>S</i>. Typhimurium (SL1344) grown in DMEM or DMEM supplemented with ethanolamine (EA) as indicated. For (<b>A</b>) and (<b>B</b>), n = 3; error bars represent the geometric mean ± SD. Statistical significance is shown relative to cells grown without EA supplementation; <i>strB</i> was used as the endogenous control. (<b>C</b>) Invasion of HeLa cells by WT (SL1344) and the Δ<i>eutR</i> (CJA009) strains. Mean ± SE of nine independent experiments. (<b>D</b>) Invasion of HeLa cells by WT (SL1344) and the Δ<i>eutR</i> (CJA009) strains. Mean ± SE of six independent experiments with supplementation of 5 mM EA. **, <i>P</i> ≤ 0.005; <i>P</i> > 0.05 = ns.