Additional file 10: of EWS and FUS bind a subset of transcribed genes encoding proteins enriched in RNA regulatory functions

Consequences of siRNA mediated depletion of FUS and EWS for gene expression. HEK-293 cells were double-transfected with specific siRNAs for FUS, EWS and FUS plus EWS, and as well as with a control siRNA (siControl). A. siRNA-depleted cells were used for relative mRNA quantification of FUS and EWS by qPCR. FUS and EWS expression was normalized to reference gene TBP. Experiments were performed in triplicates. Data presented as mean + SEM. Similar qPCR expression analyses showed a 1.5 to 2 fold increase in TAF15 mRNA by EWS and FUS depletion (not shown). B. Protein quantification by western blot of FUS and EWS from siRNA and control transfected cells. 4 F4 antibody recognizing HNRNP C1 + C2 was used as a loading control. (PDF 276 kb