T3-bound TRs negatively regulate the human D2 promoter activity induced by GATAs.

<p>(A) Using the calcium phosphate method, 2.0 μg hD2-CAT (wild-type) was transfected into CV1 cells along with the expression plasmids for TRβ2 (0.2 μg) and GATA2 (0.1 μg). *, P<0.05 for T3 (-) vs. T3 (+). (B) The inhibition by T3-bound TRβ2 is dominant over the synergism between GATA2 and PKA signaling. *, P<0.05 for vehicle (lane 1) vs. forskolin (FSK) and/or GATA2. #, P<0.05. (C) Using lipofection, hD2-hRluc (wild-type) was transfected into GH3 cells along with the expression plasmid for GATA2 (0.1 μg) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0142400#pone.0142400.g004" target="_blank">Fig 4</a>. *, P<0.05. (D) Transactivation by GATA4 but not Nkx-2.5 was repressed by T3-bound TRα1. Using the calcium phosphate method, 2.0 μg hD2-CAT (wild-type) was transfected into CV1 cells along with the expression plasmid for TRα1 (0.2 μg), FLAG-tagged Nkx-2.5 (0.1 μg) or FLAG-tagged GATA4 (0.1 μg). *, P<0.05 for the empty vector vs. pcDNA3-mGATA2. #, P<0.05. for T3 (-) vs. T3 (+).