Depletion of single SWI/SNF complex components inhibits AML maintenance.

<p>(A) Mouse MLL-AF9 cell number fold change between day 5 and day 9 after transduction. Cells were transduced with pLKO constructs expressing the indicated shRNAs and selected with puromycin from day 2 after transduction. (B) Relative mRNA levels of <i>Smarca4</i>, <i>Smarcd2</i> and <i>Dpf2</i> in MEFs transduced with the indicated pLKO constructs. (C) Number of colonies generated by MLL-AF9 cells transduced with the indicated pMLS vectors. (D) Relative mRNA levels of <i>Smarca4</i>, <i>Smarcd2</i> and <i>Dpf2</i> in MEFs transduced with the indicated pMLS vectors. (E) Rescue experiments. MLL-AF9 cells were co-transduced with pMLS-YFP carrying shScr, shSmarca4, shSmarcd2 or shDpf2, as indicated, and control pMIGRI (pMIG-Stuffer) or pMIGRI expressing human <i>SMARCA4</i>, <i>SMARCD2</i> or <i>DPF2</i> cDNA. Normalized ratios of GFP⁺/YFP⁺ cell percentages between shSmarca4, shSmarcd2 or shDpf2 and shScr samples are plotted over a time course starting from day 2 after transduction. (F) Competitive proliferation assay of c-Kit-enriched mouse bone marrow cells transduced with the indicated pMLS vectors. (G) Left: Number of colonies generated by LSK cells transduced with the indicated pLKO shRNAs. Right: Absolute numbers of LSK cells with indicated knockdown in liquid culture. (H) Survival curves of sublethally irradiated mice transplanted with 10⁴ MLL-AF9 cells transduced with the indicated pMLS vectors.