Kinetics of luciferase activity reduction after treatment with TB drugs.

<p>Three days after culturing rBCG-MDP1-luc, TB drugs were added at the same concentrations as depicted in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0141658#pone.0141658.g002" target="_blank">Fig 2</a>. At each time point, 50 μl of bacterial suspension were mixed with the same volume of luciferin-ATP mixture (Promega) and the luciferase activity was determined immediately as the RLU. At the same time, the bacterial culture was serially diluted and inoculated on 7H11-OADC agar containing 10 μg/ml of Km. Three weeks after cultivation at 37°C, the CFU were counted and compared with the RLU. The representative data of two independent experiments are presented as the means ±SD.