Coomassie stained SDS-PAGE gel analysis of point mutation purified chimeric proteins.

<p>All gels were loaded in the same manner. Lane 1: Dual color protein ladder (BioRad, Hercules, CA), Lane 2: His column purified <i>A</i>. <i>vitis</i> polygalacturonase positive control, Lane 3: His column purification flow-through, Lane 4: His column purification wash, Lanes 5–8: His column elutions notice lane 6 (Elution 2) containing chimeric protein bands denoted by arrows. Lane 9, if applicable, dual color protein ladder (BioRad, Hercules, CA).